› 3cd3

X-ray diffraction
1.98Å resolution

Crystal structure of phosphorylated human feline sarcoma viral oncogene homologue (v-FES) in complex with staurosporine and a consensus peptide

Released:
DOI: 10.2210/pdb3cd3/pdb
PDB entry 3cd3 coloured by chain, front view.
PDB entry 3cd3 coloured by chain, side view.
PDB entry 3cd3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural coupling of SH2-kinase domains links Fes and Abl substrate recognition and kinase activation.
Filippakopoulos P, Kofler M, Hantschel O, Gish GD, Grebien F, Salah E, Neudecker P, Kay LE, Turk BE, Superti-Furga G, Pawson T, Knapp S
Cell 134 793-803 (2008)
PMID: 18775312

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139509 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosine-protein kinase Fes/Fps Chain: A
Molecule details ›
Chain: A
Length: 377 amino acids
Theoretical weight: 42.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P07332 (Residues: 448-822; Coverage: 46%)
Gene names: FES, FPS
Sequence domains:
Structure domains:
Synthetic peptide Chain: B
Molecule details ›
Chain: B
Length: 6 amino acids
Theoretical weight: 650 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand CL 2 x CL
Ligand STU 3 x STU
1 modified residue:
Ligand PTR 1 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P212121
Unit cell:
a: 35.451Å b: 76.905Å c: 150.632Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.185 0.247
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

29 review citations

Protein kinases: evolution of dynamic regulatory proteins.
Taylor et al. (2011)
28 more

6 mentions without citation

Catalytic control in the EGF receptor and its connection to general kinase regulatory mechanisms.
Jura et al. (2011)
5 more