› 3c50

X-ray diffraction
2.6Å resolution

Crystal Structure of G protein coupled receptor kinase 1 bound to ADP and magnesium chloride at 2.6A

Released:
DOI: 10.2210/pdb3c50/pdb
PDB entry 3c50 coloured by chain, front view.
PDB entry 3c50 coloured by chain, side view.
PDB entry 3c50 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Structures of rhodopsin kinase in different ligand states reveal key elements involved in G protein-coupled receptor kinase activation.
Singh P, Wang B, Maeda T, Palczewski K, Tesmer JJ
J Biol Chem 283 14053-62 (2008)
PMID: 18339619

Function and Biology Details

Reaction catalysed: 
ATP + [rhodopsin] = ADP + [rhodopsin] phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151206 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rhodopsin kinase GRK1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 543 amino acids
Theoretical weight: 61.43 KDa
Source organism: Bos taurus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P28327 (Residues: 1-535; Coverage: 95%)
Gene names: GRK1, RHOK
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand MG 4 x MG
Ligand CL 8 x CL
Ligand ADP 2 x ADP
Ligand MES 1 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 65.504Å b: 122.304Å c: 174.076Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.19 not available
Expression system: Trichoplusia ni

Quick links

Citations

17 review citations

G protein-coupled receptor kinases: more than just kinases and not only for GPCRs.
Gurevich et al. (2012)
16 more

8 mentions without citation

Discovery of new GPCR biology: one receptor structure at a time.
Hanson et al. (2009)
7 more