› 3c0m

X-ray diffraction
2.88Å resolution

Crystal structure of the proaerolysin mutant Y221G

Released:
DOI: 10.2210/pdb3c0m/pdb
PDB entry 3c0m coloured by chain, front view.
PDB entry 3c0m coloured by chain, side view.
PDB entry 3c0m coloured by chain, top view.
Source organism: Aeromonas hydrophila
Primary publication:
Molecular assembly of the aerolysin pore reveals a swirling membrane-insertion mechanism.
Degiacomi MT, Iacovache I, Pernot L, Chami M, Kudryashev M, Stahlberg H, van der Goot FG, Dal Peraro M
Nat Chem Biol 9 623-9 (2013)
PMID: 23912165

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140580 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aerolysin Chains: A, B
Molecule details ›
Chains: A, B
Length: 470 amino acids
Theoretical weight: 51.87 KDa
Source organism: Aeromonas hydrophila
Expression system: Aeromonas salmonicida
UniProt:
  • Canonical: P09167 (Residues: 24-493; Coverage: 100%)
Gene name: aerA
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM1A
Spacegroup: P212121
Unit cell:
a: 72.618Å b: 92.434Å c: 169.049Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.199 0.252
Expression system: Aeromonas salmonicida

Quick links

Citations

26 review citations

Pore-forming toxins: ancient, but never really out of fashion.
Dal Peraro et al. (2016)
25 more

1 mention without citation

Structural Basis of the Pore-Forming Toxin/Membrane Interaction.
Li et al. (2021)