› 3bzn

X-ray diffraction
2Å resolution

Crystal Structure of Open form of Menaquinone-Specific Isochorismate Synthase, MenF

Released:
DOI: 10.2210/pdb3bzn/pdb
PDB entry 3bzn coloured by chain, front view.
PDB entry 3bzn coloured by chain, side view.
PDB entry 3bzn coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure of isochorismate synthase in complex with magnesium.
Parsons JF, Shi KM, Ladner JE
Acta Crystallogr D Biol Crystallogr 64 607-10 (2008)
PMID: 18453696

Function and Biology Details

Reaction catalysed: 
Chorismate = isochorismate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153534 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isochorismate synthase MenF Chain: A
Molecule details ›
Chain: A
Length: 431 amino acids
Theoretical weight: 48.82 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P38051 (Residues: 1-431; Coverage: 100%)
Gene names: JW2260, b2265, menF, yfbA
Sequence domains: chorismate binding enzyme
Structure domains: Anthranilate synthase

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P212121
Unit cell:
a: 65.28Å b: 70.49Å c: 90.28Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.202 0.284
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 citation in other articles

Crystal structure of Escherichia coli enterobactin-specific isochorismate synthase (EntC) bound to its reaction product isochorismate: implications for the enzyme mechanism and differential activity of chorismate-utilizing enzymes.
Sridharan et al. (2010)
4 more

4 mentions without citation

Nonribosomal peptides for iron acquisition: pyochelin biosynthesis as a case study.
Ronnebaum et al. (2018)
3 more