› 3bze

X-ray diffraction
2.5Å resolution

The human non-classical major histocompatibility complex molecule HLA-E

Released:
DOI: 10.2210/pdb3bze/pdb
PDB entry 3bze coloured by chain, front view.
PDB entry 3bze coloured by chain, side view.
PDB entry 3bze coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Subtle changes in peptide conformation profoundly affect recognition of the non-classical MHC class I molecule HLA-E by the CD94-NKG2 natural killer cell receptors.
Hoare HL, Sullivan LC, Clements CS, Ely LK, Beddoe T, Henderson KN, Lin J, Reid HH, Brooks AG, Rossjohn J
J Mol Biol 377 1297-303 (2008)
PMID: 18339401

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-146745 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Soluble HLA class I histocompatibility antigen, alpha chain E Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 273 amino acids
Theoretical weight: 31.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P13747 (Residues: 23-295; Coverage: 81%)
Gene names: HLA-6.2, HLA-E, HLAE
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Soluble HLA class I histocompatibility antigen, alpha chain G Chains: P, Q, R, S
Molecule details ›
Chains: P, Q, R, S
Length: 9 amino acids
Theoretical weight: 1.05 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P17693 (Residues: 3-11; Coverage: 0%)
Gene names: HLA-6.0, HLA-G, HLAG

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P21
Unit cell:
a: 104.778Å b: 73.354Å c: 131.535Å
α: 90° β: 112.7° γ: 90°
R-values:
R R work R free
0.245 0.242 0.296
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

16 review citations

The adaptable major histocompatibility complex (MHC) fold: structure and function of nonclassical and MHC class I-like molecules.
Adams et al. (2013)
15 more

4 mentions without citation

CD94-NKG2A recognition of human leukocyte antigen (HLA)-E bound to an HLA class I leader sequence.
Petrie et al. (2008)
3 more