› 3buq

X-ray diffraction
2.01Å resolution

Golgi alpha-mannosidase II D204A catalytic nucleophile mutant with bound mannose.

Released:
DOI: 10.2210/pdb3buq/pdb
PDB entry 3buq coloured by chain, front view.
PDB entry 3buq coloured by chain, side view.
PDB entry 3buq coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Probing the substrate specificity of Golgi alpha-mannosidase II by use of synthetic oligosaccharides and a catalytic nucleophile mutant.
Zhong W, Kuntz DA, Ember B, Singh H, Moremen KW, Rose DR, Boons GJ
J Am Chem Soc 130 8975-83 (2008)
PMID: 18558690

Function and Biology Details

Reaction catalysed: 
Man(5)GlcNAc(3)-[protein] + 2 H(2)O = Man(3)GlcNAc(3)-[protein] + 2 alpha-D-mannopyranose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173193 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:

Ligands and Environments

4 bound ligands:
Ligand NAG 1 x NAG
Ligand MAN 1 x MAN
Ligand ZN 1 x ZN
Ligand MPD 1 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 68.607Å b: 109.508Å c: 138.607Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.155 0.154 0.174
Expression system: Drosophila melanogaster

Quick links

Citations

1 review citation

Structure, mechanism and inhibition of Golgi α-mannosidase II.
Rose DR. (2012)
25 other articles

1 mention without citation

Probing the substrate specificity of Golgi alpha-mannosidase II by use of synthetic oligosaccharides and a catalytic nucleophile mutant.
Zhong et al. (2008)