› 3bs6

X-ray diffraction
1.8Å resolution

1.8 Angstrom crystal structure of the periplasmic domain of the membrane insertase YidC

Released:
DOI: 10.2210/pdb3bs6/pdb
PDB entry 3bs6 coloured by chain, front view.
PDB entry 3bs6 coloured by chain, side view.
PDB entry 3bs6 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The crystal structure of the periplasmic domain of the Escherichia coli membrane protein insertase YidC contains a substrate binding cleft.
Ravaud S, Stjepanovic G, Wild K, Sinning I
J Biol Chem 283 9350-8 (2008)
PMID: 18234665

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150566 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Membrane protein insertase YidC Chains: A, B
Molecule details ›
Chains: A, B
Length: 280 amino acids
Theoretical weight: 30.88 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P25714 (Residues: 56-329; Coverage: 50%)
Gene names: JW3683, b3705, yidC
Sequence domains: YidC periplasmic domain
Structure domains: Beta-galactosidase; Chain A, domain 5

Ligands and Environments

5 bound ligands:
Ligand CA 5 x CA
Ligand PG4 1 x PG4
Ligand PGE 3 x PGE
Ligand EDO 7 x EDO
Ligand 2PE 1 x 2PE
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2
Unit cell:
a: 161.15Å b: 55.64Å c: 63.33Å
α: 90° β: 101.12° γ: 90°
R-values:
R R work R free
0.179 0.178 0.213
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Signal recognition particle: an essential protein-targeting machine.
Akopian et al. (2013)
11 more

2 mentions without citation

The Sec System: Protein Export in Escherichia coli.
Crane et al. (2017)
1 more