› 3brj

X-ray diffraction
2.75Å resolution

Crystal structure of mannitol operon repressor (MtlR) from Vibrio parahaemolyticus RIMD 2210633

Released:
DOI: 10.2210/pdb3brj/pdb
PDB entry 3brj coloured by chain, front view.
PDB entry 3brj coloured by chain, side view.
PDB entry 3brj coloured by chain, top view.
Source organism: Vibrio parahaemolyticus RIMD 2210633
Primary publication:
The mannitol operon repressor MtlR belongs to a new class of transcription regulators in bacteria.
Tan K, Clancy S, Borovilos M, Zhou M, Hörer S, Moy S, Volkart LL, Sassoon J, Baumann U, Joachimiak A
J Biol Chem 284 36670-36679 (2009)
PMID: 19840941

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183373 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mannitol operon repressor Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 175 amino acids
Theoretical weight: 19.84 KDa
Source organism: Vibrio parahaemolyticus RIMD 2210633
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q87SQ4 (Residues: 1-172; Coverage: 98%)
Gene name: VP0368
Sequence domains: Mannitol repressor
Structure domains: Nucleotidyltransferases domain 2

Ligands and Environments

2 bound ligands:
Ligand EDO 4 x EDO
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P41
Unit cell:
a: 63.281Å b: 63.281Å c: 227.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.207 0.273
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

Challenges in enzymatic route of mannitol production.
Bhatt et al. (2013)
7 other articles

4 mentions without citation

Comprehensive analysis of the HEPN superfamily: identification of novel roles in intra-genomic conflicts, defense, pathogenesis and RNA processing.
Anantharaman et al. (2013)
3 more