› 3brd

X-ray diffraction
2.21Å resolution

CSL (Lag-1) bound to DNA with Lin-12 RAM peptide, P212121

Released:
DOI: 10.2210/pdb3brd/pdb
PDB entry 3brd coloured by chain, front view.
PDB entry 3brd coloured by chain, side view.
PDB entry 3brd coloured by chain, top view.
Source organism: Caenorhabditis elegans
Primary publication:
RAM-induced allostery facilitates assembly of a notch pathway active transcription complex.
Friedmann DR, Wilson JJ, Kovall RA
J Biol Chem 283 14781-91 (2008)
PMID: 18381292

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116102 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Suppressor of hairless protein homolog Chain: A
Molecule details ›
Chain: A
Length: 477 amino acids
Theoretical weight: 53.76 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli
UniProt:
  • Canonical: V6CLJ5 (Residues: 309-780; Coverage: 60%)
Gene names: K08B4.1, lag-1
Sequence domains:
Structure domains:
Protein lin-12 Chain: D
Molecule details ›
Chain: D
Length: 29 amino acids
Theoretical weight: 3.52 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli
UniProt:
  • Canonical: P14585 (Residues: 930-957; Coverage: 2%)
Gene names: R107.8, lin-12
DNA (5'-D(*DTP*DTP*DAP*DCP*DTP*DGP*DTP*DGP*DGP*DGP*DAP*DAP*DAP*DGP*DA)-3') Chain: B
Molecule details ›
Chain: B
Length: 15 nucleotides
Theoretical weight: 4.67 KDa
DNA (5'-D(*DAP*DAP*DTP*DCP*DTP*DTP*DTP*DCP*DCP*DCP*DAP*DCP*DAP*DGP*DT)-3') Chain: C
Molecule details ›
Chain: C
Length: 15 nucleotides
Theoretical weight: 4.5 KDa

Ligands and Environments

1 bound ligand:
Ligand EDO 10 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 60.153Å b: 98.866Å c: 126.313Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.208 0.257
Expression system: Escherichia coli

Quick links

Citations

10 review citations

The canonical Notch signaling pathway: unfolding the activation mechanism.
Kopan et al. (2009)
9 more

10 mentions without citation

The molecular logic of Notch signaling--a structural and biochemical perspective.
Gordon et al. (2008)
9 more