3bow Summary


Structure of M-calpain in complex with Calpastatin

The structure was published by Hanna, R.A., Campbell, R.L., and Davies, P.L., in 2008 in a paper entitled "Calcium-bound structure of calpain and its mechanism of inhibition by calpastatin." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.4 Å and deposited in 2007.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 3 biomacromolecules, namely Calpain-2 catalytic subunit, Calpain small subunit 1, and Calpastatin.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule most likely forms heterotrimers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Calpain-2 catalytic subunit Q07009 (1-700) (CAN2_RAT)search Rattus norvegicussearch 100% 714 95%
B Calpain small subunit 1 Q64537 (88-270) (CPNS1_RAT)search Rattus norvegicussearch < 90% 184 94%
C Calpastatin P27321 (571-664) (ICAL_RAT)search Rattus norvegicussearch < 90% 95 68%

This entry contains 3 unique UniProt proteins:

UniProt accession Name Organism PDB
Q07009 (1 - 700) Calpain-2 catalytic subunit Rattus norvegicus
Q64537 (88 - 270) Calpain small subunit 1 Rattus norvegicus
P27321 (571 - 664) Calpastatin Rattus norvegicus

Chain Structural classification (CATH) Sequence family (Pfam)
A (Q07009) Jelly Rollssearch, EF-handsearch PF00648: Calpain family cysteine proteasesearch, PF01067: Calpain large subunit, domain IIIsearch, PF13833: EF-hand domain pairsearch
B EF-handsearch EF handsearch, EF-hand domain pairsearch
C Calpain inhibitorsearch

Chain ID Biological process (GO) Cellular component (GO) Molecular function (GO)
A (Q07009) proteolysissearch protein autoprocessingsearch response to hypoxiasearch regulation of cytoskeleton organizationsearch blastocyst developmentsearch cellular response to amino acid stimulussearch myoblast fusionsearch intracellularsearch Golgi apparatussearch cytoplasmsearch cytosolsearch endoplasmic reticulumsearch plasma membranesearch membrane raftsearch perinuclear endoplasmic reticulumsearch extracellular vesicular exosomesearch chromatinsearch membranesearch pseudopodiumsearch nucleussearch dendritesearch cytoskeletal protein bindingsearch calcium-dependent cysteine-type endopeptidase activitysearch hydrolase activitysearch peptidase activitysearch calcium ion bindingsearch metal ion bindingsearch protein heterodimerization activitysearch protein bindingsearch cysteine-type peptidase activitysearch
B (Q64537) proteolysissearch calcium ion bindingsearch

Chain InterPro annotation
A Peptidase C2, calpain, catalytic domainsearch EF-hand domainsearch EF-hand domain pairsearch EF-Hand 1, calcium-binding sitesearch Peptidase C2, calpain, large subunit, domain IIIsearch Peptidase C2, calpain, domain IIIsearch Peptidase C2, calpain familysearch Calpain-2search
B EF-hand domainsearch EF-hand domain pairsearch EF-Hand 1, calcium-binding sitesearch Calpain small subunit 1search
C Proteinase inhibitor I27, calpastatinsearch Calpastatinsearch