3bhu Summary


Structure of phosphorylated Thr160 CDK2/cyclin A in complex with the inhibitor meriolin 5

The structure was published by Bettayeb, K., Tirado, O.M., Marionneau-Lambot, S., et al., Endicott, J.A., Notario, V., and Meijer, L., in 2007 in a paper entitled "Meriolins, a new class of cell death inducing kinase inhibitors with enhanced selectivity for cyclin-dependent kinases" (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.3 Å and deposited in 2007.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely Cell division protein kinase 2 and Cyclin-A2.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Cell division protein kinase 2 P24941 (1-298) (CDK2_HUMAN)search Homo sapienssearch 97% 300 99%
C Cell division protein kinase 2 P24941 (1-298) (CDK2_HUMAN)search Homo sapienssearch 97% 300 99%
B Cyclin-A2 P30274 (169-430) (CCNA2_BOVIN)search Bos taurussearch < 90% 262 100%
D Cyclin-A2 P30274 (169-430) (CCNA2_BOVIN)search Bos taurussearch < 90% 262 100%

This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P24941 (1 - 298) Cell division protein kinase 2 Homo sapiens
P30274 (169 - 430) Cyclin-A2 Bos taurus

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A, C (P24941) Phosphorylase Kinase; domain 1search, Transferase(Phosphotransferase) domain 1search PF00069: Protein kinase domainsearch
B, D Cyclinsearch Cyclin A; domain 1search Cyclin, N-terminal domainsearch, Cyclin, C-terminal domainsearch

Chain ID Molecular function (GO) Cellular component (GO) Biological process (GO)
A, C (P24941) protein bindingsearch metal ion bindingsearch transferase activity, transferring phosphorus-containing groupssearch protein kinase activitysearch ATP bindingsearch protein serine/threonine kinase activitysearch nucleotide bindingsearch cyclin-dependent protein serine/threonine kinase activitysearch cyclin bindingsearch kinase activitysearch transferase activitysearch histone kinase activitysearch nucleoplasmsearch X chromosomesearch cytoplasmsearch nucleussearch condensed chromosomesearch endosomesearch cyclin-dependent protein kinase holoenzyme complexsearch centrosomesearch cytosolsearch chromosome, telomeric regionsearch Cajal bodysearch transcription factor complexsearch cytoskeletonsearch Y chromosomesearch microtubule organizing centersearch mitotic G1 DNA damage checkpointsearch DNA repairsearch Ras protein signal transductionsearch histone phosphorylationsearch G1/S transition of mitotic cell cyclesearch cellular response to nitric oxidesearch positive regulation of cell proliferationsearch positive regulation of DNA-dependent DNA replication initiationsearch protein phosphorylationsearch mitotic nuclear divisionsearch cell divisionsearch regulation of ubiquitin-protein ligase activity involved in mitotic cell cyclesearch meiotic nuclear divisionsearch centrosome duplicationsearch blood coagulationsearch phosphorylationsearch DNA replicationsearch potassium ion transportsearch cell cyclesearch G2/M transition of mitotic cell cyclesearch cellular response to DNA damage stimulussearch regulation of gene silencingsearch anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic processsearch positive regulation of transcription, DNA-templatedsearch mitotic cell cyclesearch DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrestsearch
B, D (P30274) protein kinase bindingsearch nucleussearch regulation of cyclin-dependent protein serine/threonine kinase activitysearch regulation of cell cyclesearch regulation of G2/M transition of mitotic cell cyclesearch

Chain InterPro annotation
A, C Protein kinase domainsearch Serine/threonine/dual specificity protein kinase, catalytic domainsearch Serine/threonine-protein kinase, active sitesearch Protein kinase-like domainsearch Protein kinase, ATP binding sitesearch
B, D Cyclin, C-terminal domainsearch Cyclin, N-terminalsearch Cyclin-likesearch Cyclin A/B/D/E/Fsearch Cyclin A, chordatessearch