› 3bho

X-ray diffraction
1.8Å resolution

Crystal Structure of the 25kDa Subunit of Human Cleavage factor Im with Ap4A

Released:
DOI: 10.2210/pdb3bho/pdb
PDB entry 3bho coloured by chain, front view.
PDB entry 3bho coloured by chain, side view.
PDB entry 3bho coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the 25 kDa subunit of human cleavage factor Im.
Coseno M, Martin G, Berger C, Gilmartin G, Keller W, Doublié S
Nucleic Acids Res 36 3474-83 (2008)
PMID: 18445629

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129133 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cleavage and polyadenylation specificity factor subunit 5 Chain: A
Molecule details ›
Chain: A
Length: 208 amino acids
Theoretical weight: 24.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O43809 (Residues: 20-227; Coverage: 92%)
Gene names: CFIM25, CPSF25, CPSF5, NUDT21
Sequence domains: Nucleotide hydrolase
Structure domains: Nucleoside Triphosphate Pyrophosphohydrolase

Ligands and Environments

1 bound ligand:
Ligand B4P 1 x B4P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P3121
Unit cell:
a: 79.955Å b: 79.955Å c: 72.186Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.202 0.202 0.225
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Pre-mRNA 3'-end processing complex assembly and function.
Chan et al. (2011)
6 more

5 mentions without citation

Delineating the structural blueprint of the pre-mRNA 3'-end processing machinery.
Xiang et al. (2014)
4 more