› 3bgw

X-ray diffraction
3.91Å resolution

The Structure Of A DnaB-Like Replicative Helicase And Its Interactions With Primase

Released:
DOI: 10.2210/pdb3bgw/pdb
PDB entry 3bgw coloured by chain, front view.
PDB entry 3bgw coloured by chain, side view.
PDB entry 3bgw coloured by chain, top view.
Source organism: Bacillus phage SPP1
Primary publication:
The structure of a DnaB-family replicative helicase and its interactions with primase.
Wang G, Klein MG, Tokonzaba E, Zhang Y, Holden LG, Chen XS
Nat Struct Mol Biol 15 94-100 (2008)
PMID: 18157148

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo hexamer
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174316 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA helicase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 444 amino acids
Theoretical weight: 50.01 KDa
Source organism: Bacillus phage SPP1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q38152 (Residues: 1-442; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P212121
Unit cell:
a: 114.634Å b: 184.411Å c: 184.47Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.339 0.338 0.349
Expression system: Escherichia coli

Quick links

Citations

12 review citations

The Mcm complex: unwinding the mechanism of a replicative helicase.
Bochman et al. (2009)
11 more

4 mentions without citation

Integrating ion mobility mass spectrometry with molecular modelling to determine the architecture of multiprotein complexes.
Politis et al. (2010)
3 more