› 3bdx

X-ray diffraction
2.3Å resolution

Crystal structure of the unstable and highly fibrillogenic Pro7Ser mutant of the Recombinant variable domain 6AJL2

Released:
DOI: 10.2210/pdb3bdx/pdb
PDB entry 3bdx coloured by chain, front view.
PDB entry 3bdx coloured by chain, side view.
PDB entry 3bdx coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A single mutation at the sheet switch region results in conformational changes favoring lambda6 light-chain fibrillogenesis.
Hernández-Santoyo A, del Pozo Yauner L, Fuentes-Silva D, Ortiz E, Rudiño-Piñera E, Sánchez-López R, Horjales E, Becerril B, Rodríguez-Romero A
J Mol Biol 396 280-92 (2010)
PMID: 19941869

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo trimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188571 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ig-like domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 111 amino acids
Theoretical weight: 11.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96JD1 (Residues: 1-111; Coverage: 99%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

3 bound ligands:
Ligand ACT 5 x ACT
Ligand GOL 4 x GOL
Ligand MES 1 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2221
Unit cell:
a: 56.11Å b: 106.12Å c: 133.36Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.205 0.246
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Amyloid formation in light chain amyloidosis.
Ramirez-Alvarado M. (2012)
4 more

5 mentions without citation

Structure of the three N-terminal immunoglobulin domains of the highly immunogenic outer capsid protein from a T4-like bacteriophage.
Fokine et al. (2011)
4 more