› 3bb4

X-ray diffraction
2.85Å resolution

Crystal structure of Toc33 from Arabidopsis thaliana in complex with Mg2+ and GMPPNP

Released:
DOI: 10.2210/pdb3bb4/pdb
PDB entry 3bb4 coloured by chain, front view.
PDB entry 3bb4 coloured by chain, side view.
PDB entry 3bb4 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
The GTPase cycle of the chloroplast import receptors Toc33/Toc34: implications from monomeric and dimeric structures.
Koenig P, Oreb M, Höfle A, Kaltofen S, Rippe K, Sinning I, Schleiff E, Tews I
Structure 16 585-96 (2008)
PMID: 18400179

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127655 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Translocase of chloroplast 33, chloroplastic Chain: A
Molecule details ›
Chain: A
Length: 262 amino acids
Theoretical weight: 29.26 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O23680 (Residues: 1-251; Coverage: 85%)
Gene names: At1g02280, PPI1, T7I23.11, TOC33
Sequence domains: AIG1 family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand GNP 1 x GNP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P41212
Unit cell:
a: 121.567Å b: 121.567Å c: 42.879Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.218 0.277
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

14 review citations

Structure-function relationships of the G domain, a canonical switch motif.
Wittinghofer et al. (2011)
13 more

1 mention without citation

The effect of the surgical microscope on the outcome of root scaling.
Liao et al. (2020)