3b8w Summary


Crystal structure of Escherichia coli alaine racemase mutant E221P

The structure was published by Wu, D., Hu, T., Zhang, L., et al., Ding, J., Jiang, H., and Shen, X., in 2008 in a paper entitled "Residues Asp164 and Glu165 at the substrate entryway function potently in substrate orientation of alanine racemase from E. coli: Enzymatic characterization with crystal structure analysis" (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.7 Å and deposited in 2007.

The experimental data on which the structure is based was also deposited.

This PDB entry contains multiple copies of the structure of Alanine racemase.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Alanine racemase P0A6B4 (1-359) (ALR1_ECOLI)search Escherichia coli K-12search 100% 379 94%
B Alanine racemase P0A6B4 (1-359) (ALR1_ECOLI)search Escherichia coli K-12search 100% 379 94%
C Alanine racemase P0A6B4 (1-359) (ALR1_ECOLI)search Escherichia coli K-12search 100% 379 94%
D Alanine racemase P0A6B4 (1-359) (ALR1_ECOLI)search Escherichia coli K-12search 100% 379 94%

This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
P0A6B4 (1 - 359) Alanine racemase Escherichia coli K-12

Chain Structural classification (CATH) Sequence family (Pfam)
A, B, C, D (P0A6B4) Lyase, Ornithine Decarboxylase; Chain A, domain 1search, Alanine racemasesearch PF00842: Alanine racemase, C-terminal domainsearch, PF01168: Alanine racemase, N-terminal domainsearch

Chain ID Molecular function (GO) Biological process (GO)
A, B, C, D (P0A6B4) alanine racemase activitysearch isomerase activitysearch pyridoxal phosphate bindingsearch catalytic activitysearch peptidoglycan biosynthetic processsearch alanine metabolic processsearch D-alanine biosynthetic processsearch regulation of cell shapesearch cell wall organizationsearch

Chain InterPro annotation
A, B, C, D Alanine racemasesearch Alanine racemase, N-terminalsearch Alanine racemase/group IV decarboxylase, C-terminalsearch Alanine racemase, C-terminalsearch Alanine racemase, pyridoxal-phosphate attachment sitesearch PLP-binding barrelsearch