› 3b7c

X-ray diffraction
1.7Å resolution

CRYSTAL STRUCTURE OF A NTF-2 LIKE PROTEIN OF UNKNOWN FUNCTION (SO_0125) FROM SHEWANELLA ONEIDENSIS MR-1 AT 1.70 A RESOLUTION

Released:
DOI: 10.2210/pdb3b7c/pdb
PDB entry 3b7c coloured by chain, front view.
PDB entry 3b7c coloured by chain, side view.
PDB entry 3b7c coloured by chain, top view.
Source organism: Shewanella oneidensis MR-1
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
L-asparagine + H(2)O = L-aspartate + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184421 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DUF4440 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 122 amino acids
Theoretical weight: 14.48 KDa
Source organism: Shewanella oneidensis MR-1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8EKG8 (Residues: 29-149; Coverage: 100%)
Gene name: SO_0125
Sequence domains: Domain of unknown function (DUF4440)
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

2 bound ligands:
Ligand CL 1 x CL
Ligand EDO 6 x EDO
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P6522
Unit cell:
a: 66.19Å b: 66.19Å c: 126.07Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.186 0.184 0.214
Expression system: Escherichia coli

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