› 3b6a

X-ray diffraction
3.05Å resolution

Crystal structure of the Streptomyces coelicolor TetR family protein ActR in complex with actinorhodin

Released:
DOI: 10.2210/pdb3b6a/pdb
PDB entry 3b6a coloured by chain, front view.
PDB entry 3b6a coloured by chain, side view.
PDB entry 3b6a coloured by chain, top view.
Source organism: Streptomyces coelicolor
Primary publication:
Crystal structures of the Streptomyces coelicolor TetR-like protein ActR alone and in complex with actinorhodin or the actinorhodin biosynthetic precursor (S)-DNPA.
Willems AR, Tahlan K, Taguchi T, Zhang K, Lee ZZ, Ichinose K, Junop MS, Nodwell JR
J Mol Biol 376 1377-87 (2008)
PMID: 18207163

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176083 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH tetR-type domain-containing protein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 234 amino acids
Theoretical weight: 25.2 KDa
Source organism: Streptomyces coelicolor
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q53901 (Residues: 30-259; Coverage: 89%)
Gene name: actII
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZCT 8 x ZCT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P1
Unit cell:
a: 57.88Å b: 79Å c: 107.19Å
α: 89.56° β: 89.92° γ: 89.88°
R-values:
R R work R free
0.261 0.26 0.288
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

The regulation of the secondary metabolism of Streptomyces: new links and experimental advances.
van Wezel et al. (2011)
11 more

4 mentions without citation

The TetR family of regulators.
Cuthbertson et al. (2013)
3 more