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Membrane-bound respiratory [NiFe] hydrogenase from Hydrogenovibrio marinus in an H2-reduced condition

X-ray diffraction
1.18Å resolution
PDB entry 3ayx coloured by chain, front view.
PDB entry 3ayx coloured by chain, side view.
PDB entry 3ayx coloured by chain, top view.
Source organism: Hydrogenovibrio marinus
Assembly composition:
hetero tetramer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: hydrogenase (acceptor) and Uptake hydrogenase large subunit
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Multimeric state: hetero tetramer
Accessible surface area: 48473.45 Å2
Buried surface area: 25766.49 Å2
Dissociation area: 2,672.23 Å2
Dissociation energy (ΔGdiss): 28.25 kcal/mol
Dissociation entropy (TΔSdiss): 16.08 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-123456

Macromolecules

Uptake hydrogenase large subunit
Chains: A, C
Length: 596 amino acids
Theoretical weight: 66.57 KDa
Source organism: Hydrogenovibrio marinus
UniProt:
  • Canonical: F2Z6J6 (Residues: 1-596; Coverage: 98%)
Gene names: EI16_00020, hoxG
Pfam: Nickel-dependent hydrogenase
InterPro:
CATH: Cytochrome-c3 Hydrogenase, chain B
Membrane-bound hydrogenase large subunit in PDB entry 3ayx, assembly 1, front view.
Membrane-bound hydrogenase large subunit in PDB entry 3ayx, assembly 1, side view.
Membrane-bound hydrogenase large subunit in PDB entry 3ayx, assembly 1, top view.
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hydrogenase (acceptor)
Chains: B, D
Length: 283 amino acids
Theoretical weight: 31.31 KDa
Source organism: Hydrogenovibrio marinus
UniProt:
  • Canonical: F2Z6J5 (Residues: 41-323; Coverage: 78%)
Gene name: hoxK
Pfam:
InterPro:
CATH:
hydrogenase (acceptor) in PDB entry 3ayx, assembly 1, front view.
hydrogenase (acceptor) in PDB entry 3ayx, assembly 1, side view.
hydrogenase (acceptor) in PDB entry 3ayx, assembly 1, top view.
Molecule details ›

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