› 3am6

X-ray diffraction
3.2Å resolution

Crystal structure of the proton pumping rhodopsin AR2 from marine alga Acetabularia acetabulum

Released:
DOI: 10.2210/pdb3am6/pdb
PDB entry 3am6 coloured by chain, front view.
PDB entry 3am6 coloured by chain, side view.
PDB entry 3am6 coloured by chain, top view.
Source organism: Acetabularia acetabulum
Primary publication:
Crystal structure of the eukaryotic light-driven proton-pumping rhodopsin, Acetabularia rhodopsin II, from marine alga.
Wada T, Shimono K, Kikukawa T, Hato M, Shinya N, Kim SY, Kimura-Someya T, Shirouzu M, Tamogami J, Miyauchi S, Jung KH, Kamo N, Yokoyama S
J Mol Biol 411 986-98 (2011)
PMID: 21726566

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-124189 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rhodopsin-2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 229 amino acids
Theoretical weight: 25.56 KDa
Source organism: Acetabularia acetabulum
Expression system: cell-free synthesis
UniProt:
  • Canonical: G1K3Q0 (Residues: 1-229; Coverage: 100%)
Sequence domains: Bacteriorhodopsin-like protein
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

2 bound ligands:
Ligand RET 4 x RET
Ligand CLR 8 x CLR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 84.12Å b: 110.487Å c: 129.116Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.29 0.29 0.324
Expression system: cell-free synthesis

Quick links

Citations

14 review citations

Microbial and animal rhodopsins: structures, functions, and molecular mechanisms.
Ernst et al. (2014)
13 more

11 mentions without citation

A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.
Caffrey M. (2015)
10 more