› 3aje

X-ray diffraction
1.8Å resolution

Crystal structure of S. tokodaii Sua5 complexed with L-threonine and AMPPNP

Released:
DOI: 10.2210/pdb3aje/pdb
PDB entry 3aje coloured by chain, front view.
PDB entry 3aje coloured by chain, side view.
PDB entry 3aje coloured by chain, top view.
Source organism: Sulfurisphaera tokodaii str. 7
Primary publication:
Crystal structure of Sulfolobus tokodaii Sua5 complexed with L-threonine and AMPPNP.
Kuratani M, Kasai T, Akasaka R, Higashijima K, Terada T, Kigawa T, Shinkai A, Bessho Y, Yokoyama S
Proteins 79 2065-75 (2011)
PMID: 21538543

Function and Biology Details

Reaction catalysed: 
L-threonine + ATP + HCO(3)(-) = L-threonylcarbamoyladenylate + diphosphate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188813 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Threonylcarbamoyl-AMP synthase Chain: A
Molecule details ›
Chain: A
Length: 352 amino acids
Theoretical weight: 38.85 KDa
Source organism: Sulfurisphaera tokodaii str. 7
Expression system: Escherichia coli
UniProt:
  • Canonical: Q970S6 (Residues: 1-352; Coverage: 100%)
Gene names: STK_15260, sua5
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand THR 1 x THR
Ligand ANP 1 x ANP
Ligand NO3 6 x NO3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: C222
Unit cell:
a: 85.35Å b: 121Å c: 68.01Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.198 0.238
Expression system: Escherichia coli

Quick links

Citations

4 review citations

The O-carbamoyltransferase TobZ catalyzes an ancient enzymatic reaction.
Parthier et al. (2012)
3 more

6 mentions without citation

The Blueprint of a Minimal Cell: MiniBacillus.
Reuß et al. (2016)
5 more