› 3ac0

X-ray diffraction
2.54Å resolution

Crystal structure of Beta-glucosidase from Kluyveromyces marxianus in complex with glucose

Released:
DOI: 10.2210/pdb3ac0/pdb
PDB entry 3ac0 coloured by chain, front view.
PDB entry 3ac0 coloured by chain, side view.
PDB entry 3ac0 coloured by chain, top view.
Source organism: Kluyveromyces marxianus
Primary publication:
Role of a PA14 domain in determining substrate specificity of a glycoside hydrolase family 3 β-glucosidase from Kluyveromyces marxianus.
Yoshida E, Hidaka M, Fushinobu S, Koyanagi T, Minami H, Tamaki H, Kitaoka M, Katayama T, Kumagai H
Biochem J 431 39-49 (2010)
PMID: 20662765

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112300 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
beta-glucosidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 845 amino acids
Theoretical weight: 93.81 KDa
Source organism: Kluyveromyces marxianus
Expression system: Escherichia coli
UniProt:
  • Canonical: D1GCC6 (Residues: 1-845; Coverage: 100%)
Gene name: Bgl
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand BGC 4 x BGC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: C2
Unit cell:
a: 245.847Å b: 148.412Å c: 119.642Å
α: 90° β: 112.84° γ: 90°
R-values:
R R work R free
0.173 0.169 0.242
Expression system: Escherichia coli

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Citations

9 review citations

Xylan degradation, a metabolic property shared by rumen and human colonic Bacteroidetes.
Dodd et al. (2011)
8 more

7 mentions without citation

Development of cellobiose-degrading ability in Yarrowia lipolytica strain by overexpression of endogenous genes.
Guo et al. (2015)
6 more