3aaa Summary


Crystal Structure of Actin capping protein in complex with V-1

The structure was published by Takeda, S., Minakata, S., Koike, R., et al., Yamakuni, T., Maeda, Y., and Nitanai, Y., in 2010 in a paper entitled "Two distinct mechanisms for actin capping protein regulation--steric and allosteric inhibition" (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.2 Å and deposited in 2009.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 3 biomacromolecules, namely F-actin-capping protein subunit alpha-1, F-actin-capping protein subunit beta isoforms 1 and 2, and Myotrophin.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A F-actin-capping protein subunit alpha-1 P13127 (1-286) (CAZA1_CHICK)search Gallus gallussearch 96% 286 93%
B F-actin-capping protein subunit beta isoforms 1 and 2 P14315 (1-277) (CAPZB_CHICK)search Gallus gallussearch 97% 277 90%
C Myotrophin P58546 (1-118) (MTPN_HUMAN)search Homo sapienssearch 100% 123 95%

This entry contains 3 unique UniProt proteins:

UniProt accession Name Organism PDB
P13127 (1 - 286) F-actin-capping protein subunit alpha-1 Gallus gallus
P14315 (1 - 277) F-actin-capping protein subunit beta isoforms 1 and 2 Gallus gallus
P58546 (1 - 118) Myotrophin Homo sapiens

Chain Structural classification (CATH) Sequence family (Pfam)
A (P13127) PF01267: F-actin capping protein alpha subunitsearch
B (P14315) PF01115: F-actin capping protein, beta subunitsearch
C (P58546) Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeatsearch PF12796: Ankyrin repeats (3 copies)search

Chain ID Cellular component (GO) Molecular function (GO) Biological process (GO)
A (P13127) extracellular vesicular exosomesearch Z discsearch cytoplasmsearch F-actin capping protein complexsearch WASH complexsearch cytosolsearch actin bindingsearch actin cytoskeleton organizationsearch actin filament cappingsearch
B (P14315) F-actin capping protein complexsearch membranesearch cytoskeletonsearch cytoplasmsearch lamellipodiumsearch WASH complexsearch cortical cytoskeletonsearch acrosomal vesiclesearch actin cortical patchsearch intercalated discsearch cytosolsearch Z discsearch actin filamentsearch I bandsearch beta-tubulin bindingsearch actin bindingsearch actin filament bindingsearch neuron projection developmentsearch barbed-end actin filament cappingsearch actin cytoskeleton organizationsearch lamellipodium assemblysearch muscle fiber developmentsearch cell projection organizationsearch negative regulation of filopodium assemblysearch regulation of cell morphogenesissearch regulation of lamellipodium assemblysearch negative regulation of microtubule polymerizationsearch actin filament cappingsearch cytoskeleton organizationsearch cell morphogenesissearch regulation of protein kinase C signalingsearch
C (P58546) cytosolsearch nucleussearch perinuclear region of cytoplasmsearch cytoplasmsearch extracellular vesicular exosomesearch F-actin capping protein complexsearch axonsearch positive regulation of cardiac muscle hypertrophysearch positive regulation of cell growthsearch regulation of barbed-end actin filament cappingsearch positive regulation of macromolecule biosynthetic processsearch positive regulation of NF-kappaB transcription factor activitysearch skeletal muscle tissue regenerationsearch catecholamine metabolic processsearch positive regulation of protein metabolic processsearch regulation of translationsearch cerebellar granule cell differentiationsearch neuron differentiationsearch striated muscle cell differentiationsearch cell growthsearch regulation of striated muscle tissue developmentsearch

Chain InterPro annotation
A F-actin-capping protein subunit alphasearch F-actin capping protein, alpha subunit, conserved sitesearch
B F-actin-capping protein subunit betasearch F-actin capping protein, beta subunit, conserved sitesearch
C Ankyrin repeatsearch Ankyrin repeat-containing domainsearch