3aaa Summary


Crystal Structure of Actin capping protein in complex with V-1

The structure was published by Takeda, S., Minakata, S., Koike, R., et al., Yamakuni, T., Maeda, Y., and Nitanai, Y., in 2010 in a paper entitled "Two distinct mechanisms for actin capping protein regulation--steric and allosteric inhibition" (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.2 Å and deposited in 2009.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 3 biomacromolecules, namely F-actin-capping protein subunit alpha-1, F-actin-capping protein subunit beta isoforms 1 and 2, and Myotrophin.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A F-actin-capping protein subunit alpha-1 P13127 (1-286) (CAZA1_CHICK)search Gallus gallussearch 96% 286 93%
B F-actin-capping protein subunit beta isoforms 1 and 2 P14315 (1-277) (CAPZB_CHICK)search Gallus gallussearch 97% 277 90%
C Myotrophin P58546 (1-118) (MTPN_HUMAN)search Homo sapienssearch 100% 123 95%

This entry contains 3 unique UniProt proteins:

UniProt accession Name Organism PDB
P13127 (1 - 286) F-actin-capping protein subunit alpha-1 Gallus gallus
P14315 (1 - 277) F-actin-capping protein subunit beta isoforms 1 and 2 Gallus gallus
P58546 (1 - 118) Myotrophin Homo sapiens

Chain Structural classification (CATH) Sequence family (Pfam)
A (P13127) PF01267: F-actin capping protein alpha subunitsearch
B (P14315) PF01115: F-actin capping protein, beta subunitsearch
C (P58546) Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeatsearch PF12796: Ankyrin repeats (3 copies)search, PF13637: Ankyrin repeats (many copies)search

Chain ID Cellular component (GO) Molecular function (GO) Biological process (GO)
A (P13127) F-actin capping protein complexsearch extracellular vesicular exosomesearch Z discsearch cytosolsearch WASH complexsearch cytoplasmsearch actin bindingsearch actin filament cappingsearch actin cytoskeleton organizationsearch
B (P14315) cytoskeletonsearch Z discsearch intercalated discsearch cortical cytoskeletonsearch cytosolsearch cytoplasmsearch I bandsearch F-actin capping protein complexsearch acrosomal vesiclesearch WASH complexsearch lamellipodiumsearch membranesearch actin bindingsearch beta-tubulin bindingsearch actin filament bindingsearch actin filament cappingsearch regulation of cell morphogenesissearch barbed-end actin filament cappingsearch negative regulation of microtubule polymerizationsearch cytoskeleton organizationsearch neuron projection developmentsearch regulation of protein kinase C signalingsearch cell projection organizationsearch muscle fiber developmentsearch lamellipodium assemblysearch actin cytoskeleton organizationsearch
C (P58546) cytosolsearch F-actin capping protein complexsearch nucleussearch axonsearch cytoplasmsearch perinuclear region of cytoplasmsearch extracellular vesicular exosomesearch neuron differentiationsearch regulation of translationsearch positive regulation of cell growthsearch positive regulation of NF-kappaB transcription factor activitysearch cell growthsearch regulation of striated muscle tissue developmentsearch positive regulation of cardiac muscle hypertrophysearch catecholamine metabolic processsearch striated muscle cell differentiationsearch positive regulation of macromolecule biosynthetic processsearch positive regulation of protein metabolic processsearch regulation of barbed-end actin filament cappingsearch cerebellar granule cell differentiationsearch skeletal muscle tissue regenerationsearch

Chain InterPro annotation
A F-actin-capping protein subunit alphasearch F-actin capping protein, alpha subunit, conserved sitesearch
B F-actin-capping protein subunit betasearch F-actin capping protein, beta subunit, conserved sitesearch
C Ankyrin repeatsearch Ankyrin repeat-containing domainsearch