3aaa Summary


Crystal Structure of Actin capping protein in complex with V-1

The structure was published by Takeda, S., Minakata, S., Koike, R., et al., Yamakuni, T., Maeda, Y., and Nitanai, Y., in 2010 in a paper entitled "Two distinct mechanisms for actin capping protein regulation--steric and allosteric inhibition" (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.2 Å and deposited in 2009.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 3 biomacromolecules, namely F-actin-capping protein subunit alpha-1, F-actin-capping protein subunit beta isoforms 1 and 2, and Myotrophin.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A F-actin-capping protein subunit alpha-1 P13127 (1-286) (CAZA1_CHICK)search Gallus gallussearch 96% 286 93%
B F-actin-capping protein subunit beta isoforms 1 and 2 P14315 (1-277) (CAPZB_CHICK)search Gallus gallussearch 100% 277 90%
C Myotrophin P58546 (1-118) (MTPN_HUMAN)search Homo sapienssearch 100% 123 95%

This entry contains 3 unique UniProt proteins:

UniProt accession Name Organism PDB
P13127 (1 - 286) F-actin-capping protein subunit alpha-1 Gallus gallus
P14315 (1 - 277) F-actin-capping protein subunit beta isoforms 1 and 2 Gallus gallus
P58546 (1 - 118) Myotrophin Homo sapiens

Chain Structural classification (CATH) Sequence family (Pfam)
A (P13127) PF01267: F-actin capping protein alpha subunitsearch
B (P14315) PF01115: F-actin capping protein, beta subunitsearch
C (P58546) Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeatsearch PF12796: Ankyrin repeats (3 copies)search

Chain ID Biological process (GO) Cellular component (GO) Molecular function (GO)
A (P13127) actin filament cappingsearch actin cytoskeleton organizationsearch cytoplasmsearch extracellular vesicular exosomesearch cytosolsearch F-actin capping protein complexsearch WASH complexsearch Z discsearch actin bindingsearch
B (P14315) cell morphogenesissearch negative regulation of filopodium assemblysearch regulation of lamellipodium assemblysearch lamellipodium assemblysearch negative regulation of microtubule polymerizationsearch regulation of protein kinase C signalingsearch barbed-end actin filament cappingsearch actin cytoskeleton organizationsearch cytoskeleton organizationsearch muscle fiber developmentsearch actin filament cappingsearch neuron projection developmentsearch cell projection organizationsearch regulation of cell morphogenesissearch cytoskeletonsearch Z discsearch actin cortical patchsearch cortical cytoskeletonsearch cytosolsearch cytoplasmsearch lamellipodiumsearch F-actin capping protein complexsearch actin filamentsearch I bandsearch acrosomal vesiclesearch membranesearch intercalated discsearch WASH complexsearch actin filament bindingsearch actin bindingsearch beta-tubulin bindingsearch
C (P58546) regulation of translationsearch skeletal muscle tissue regenerationsearch catecholamine metabolic processsearch positive regulation of cardiac muscle hypertrophysearch positive regulation of macromolecule biosynthetic processsearch cerebellar granule cell differentiationsearch cell growthsearch regulation of striated muscle tissue developmentsearch positive regulation of protein metabolic processsearch positive regulation of cell growthsearch regulation of barbed-end actin filament cappingsearch positive regulation of NF-kappaB transcription factor activitysearch striated muscle cell differentiationsearch neuron differentiationsearch cytosolsearch nucleussearch axonsearch perinuclear region of cytoplasmsearch cytoplasmsearch extracellular vesicular exosomesearch F-actin capping protein complexsearch

Chain InterPro annotation
A F-actin-capping protein subunit alphasearch F-actin capping protein, alpha subunit, conserved sitesearch
B F-actin-capping protein subunit betasearch F-actin capping protein, beta subunit, conserved sitesearch
C Ankyrin repeatsearch Ankyrin repeat-containing domainsearch