› 3a5t

X-ray diffraction
2.8Å resolution

Crystal structure of MafG-DNA complex

Released:
DOI: 10.2210/pdb3a5t/pdb
PDB entry 3a5t coloured by chain, front view.
PDB entry 3a5t coloured by chain, side view.
PDB entry 3a5t coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of alternative DNA recognition by Maf transcription factors.
Kurokawa H, Motohashi H, Sueno S, Kimura M, Takagawa H, Kanno Y, Yamamoto M, Tanaka T
Mol Cell Biol 29 6232-44 (2009)
PMID: 19797082

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116058 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transcription factor MafG Chains: A, B
Molecule details ›
Chains: A, B
Length: 107 amino acids
Theoretical weight: 12.52 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: O54790 (Residues: 21-123; Coverage: 64%)
Gene name: Mafg
Sequence domains: bZIP Maf transcription factor
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
5'-D(*CP*TP*GP*AP*TP*GP*AP*GP*TP*CP*AP*GP*CP*AP*C)-3' Chain: C
Molecule details ›
Chain: C
Length: 15 nucleotides
Theoretical weight: 4.59 KDa
Source organism: synthetic construct
Expression system: Not provided
5'-D(*GP*TP*GP*CP*TP*GP*AP*CP*TP*CP*AP*TP*CP*AP*G)-3' Chain: D
Molecule details ›
Chain: D
Length: 15 nucleotides
Theoretical weight: 4.58 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: C2221
Unit cell:
a: 40.364Å b: 185.243Å c: 230.263Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.261 0.261 0.294
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

11 review citations

The KEAP1-NRF2 System: a Thiol-Based Sensor-Effector Apparatus for Maintaining Redox Homeostasis.
Yamamoto et al. (2018)
10 more

10 mentions without citation

Small Maf proteins (MafF, MafG, MafK): History, structure and function.
Katsuoka et al. (2016)
9 more