3a3n

X-ray diffraction
2.2Å resolution

Crystal structure of complex between SA-subtilisin and Tk-propeptide with deletion of the two C-terminal residues

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157698 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tk-subtilisin Chain: A
Molecule details ›
Chain: A
Length: 329 amino acids
Theoretical weight: 33.9 KDa
Source organism: Thermococcus kodakarensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P58502 (Residues: 94-422; Coverage: 83%)
Gene name: TK1675
Sequence domains: Subtilase family
Structure domains: Peptidase S8/S53 domain
Tk-subtilisin Chain: B
Molecule details ›
Chain: B
Length: 67 amino acids
Theoretical weight: 7.29 KDa
Source organism: Thermococcus kodakarensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P58502 (Residues: 25-91; Coverage: 17%)
Gene name: TK1675
Structure domains: Peptidase S8 propeptide/proteinase inhibitor I9

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P212121
Unit cell:
a: 65.732Å b: 68.468Å c: 73.704Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.17 0.231
Expression system: Escherichia coli BL21(DE3)