› 3a23

X-ray diffraction
1.9Å resolution

Crystal Structure of beta-L-Arabinopyranosidase complexed with D-galactose

Released:
DOI: 10.2210/pdb3a23/pdb
PDB entry 3a23 coloured by chain, front view.
PDB entry 3a23 coloured by chain, side view.
PDB entry 3a23 coloured by chain, top view.
Source organism: Streptomyces avermitilis
Primary publication:
A beta-l-Arabinopyranosidase from Streptomyces avermitilis is a novel member of glycoside hydrolase family 27.
Ichinose H, Fujimoto Z, Honda M, Harazono K, Nishimoto Y, Uzura A, Kaneko S
J Biol Chem 284 25097-106 (2009)
PMID: 19608743

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182667 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-galactosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 614 amino acids
Theoretical weight: 64.31 KDa
Source organism: Streptomyces avermitilis
Expression system: Streptomyces lividans
UniProt:
  • Canonical: Q82L26 (Residues: 45-658; Coverage: 100%)
Gene names: SAVERM_2186, agaA2
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand GAL 3 x GAL
Ligand GOL 19 x GOL
Ligand 1PG 2 x 1PG
Ligand SO4 5 x SO4
Ligand EPE 1 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P212121
Unit cell:
a: 68.256Å b: 99.081Å c: 181.63Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.144 0.142 0.177
Expression system: Streptomyces lividans

Quick links

Citations

5 review citations

Arabinogalactan proteins: focus on carbohydrate active enzymes.
Knoch et al. (2014)
4 more

2 mentions without citation

A beta-l-Arabinopyranosidase from Streptomyces avermitilis is a novel member of glycoside hydrolase family 27.
Ichinose et al. (2009)
1 more