› 3zsk

X-ray diffraction
0.9Å resolution

Crystal structure of Human Galectin-3 CRD with glycerol bound at 0.90 angstrom resolution

Released:
DOI: 10.2210/pdb3zsk/pdb
PDB entry 3zsk coloured by chain, front view.
PDB entry 3zsk coloured by chain, side view.
PDB entry 3zsk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The carbohydrate-binding site in galectin-3 is preorganized to recognize a sugarlike framework of oxygens: ultra-high-resolution structures and water dynamics.
Saraboji K, Håkansson M, Genheden S, Diehl C, Qvist J, Weininger U, Nilsson UJ, Leffler H, Ryde U, Akke M, Logan DT
Biochemistry 51 296-306 (2012)
PMID: 22111949

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148215 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Galectin-3 Chain: A
Molecule details ›
Chain: A
Length: 138 amino acids
Theoretical weight: 15.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P17931 (Residues: 114-250; Coverage: 55%)
Gene names: LGALS3, MAC2
Sequence domains: Galactoside-binding lectin
Structure domains: Jelly Rolls

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-5
Spacegroup: P212121
Unit cell:
a: 35.83Å b: 58.2Å c: 62.54Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.132 not available 0.15
Expression system: Escherichia coli

Quick links

Citations

11 review citations

The dark energy of proteins comes to light: conformational entropy and its role in protein function revealed by NMR relaxation.
Wand AJ. (2013)
10 more

4 mentions without citation

The carbohydrate-binding site in galectin-3 is preorganized to recognize a sugarlike framework of oxygens: ultra-high-resolution structures and water dynamics.
Saraboji et al. (2012)
3 more