› 3zfm

X-ray diffraction
2.27Å resolution

Crystal structure of EphB2

Released:
DOI: 10.2210/pdb3zfm/pdb
PDB entry 3zfm coloured by chain, front view.
PDB entry 3zfm coloured by chain, side view.
PDB entry 3zfm coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Completing the structural family portrait of the human EphB tyrosine kinase domains.
Overman RC, Debreczeni JE, Truman CM, McAlister MS, Attwood TK
Protein Sci 23 627-38 (2014)
PMID: 24677421

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151405 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
EphB2/CTF2 Chain: A
Molecule details ›
Chain: A
Length: 298 amino acids
Theoretical weight: 33.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P29323 (Residues: 604-898; Coverage: 28%)
Gene names: DRT, EPHB2, EPHT3, EPTH3, ERK, HEK5, TYRO5
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E
Spacegroup: P1
Unit cell:
a: 34.59Å b: 41.19Å c: 54.47Å
α: 92.65° β: 97.15° γ: 114.79°
R-values:
R R work R free
0.236 0.235 0.263
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Factors involved in heterologous expression of proteins in E. coli host.
Pouresmaeil et al. (2023)
8 other articles

5 mentions without citation

Completing the structural family portrait of the human EphB tyrosine kinase domains.
Overman et al. (2014)
4 more