› 3zdt

X-ray diffraction
3.15Å resolution

Crystal structure of basic patch mutant FAK FERM domain FAK31- 405 K216A, K218A, R221A, K222A

Released:
DOI: 10.2210/pdb3zdt/pdb
PDB entry 3zdt coloured by chain, front view.
PDB entry 3zdt coloured by chain, side view.
PDB entry 3zdt coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Phosphatidylinositol 4,5-bisphosphate triggers activation of focal adhesion kinase by inducing clustering and conformational changes.
Goñi GM, Epifano C, Boskovic J, Camacho-Artacho M, Zhou J, Bronowska A, Martín MT, Eck MJ, Kremer L, Gräter F, Gervasio FL, Perez-Moreno M, Lietha D
Proc Natl Acad Sci U S A 111 E3177-86 (2014)
PMID: 25049397

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169484 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Focal adhesion kinase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 377 amino acids
Theoretical weight: 42.9 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q00944 (Residues: 31-405; Coverage: 36%)
Gene names: FAK, FAK1, PTK2
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 41.567Å b: 175.527Å c: 60.032Å
α: 90° β: 93.94° γ: 90°
R-values:
R R work R free
0.217 0.214 0.278
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

28 review citations

FAK signaling in human cancer as a target for therapeutics.
Lee et al. (2015)
27 more

2 mentions without citation

Phosphatidylinositol 4,5-bisphosphate triggers activation of focal adhesion kinase by inducing clustering and conformational changes.
Goñi et al. (2014)
1 more