› 3zc0

X-ray diffraction
2.98Å resolution

Structure of AfC3PO - duplex RNA complex

Released:
DOI: 10.2210/pdb3zc0/pdb
PDB entry 3zc0 coloured by chain, front view.
PDB entry 3zc0 coloured by chain, side view.
PDB entry 3zc0 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for duplex RNA recognition and cleavage by Archaeoglobus fulgidus C3PO.
Parizotto EA, Lowe ED, Parker JS
Nat Struct Mol Biol 20 380-6 (2013)
PMID: 23353787

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127930 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Translin Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 199 amino acids
Theoretical weight: 22.8 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O28024 (Residues: 1-196; Coverage: 100%)
Gene name: AF_2260
Sequence domains: Translin family
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1
5'-R(*UP*UP*CP*GP*AP*CP*GP*CP*GP*UP*CP*GP*AP*AP*UP*U)-3' Chains: M, N, O
Molecule details ›
Chains: M, N, O
Length: 16 nucleotides
Theoretical weight: 5.08 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MG 12 x MG
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P3121
Unit cell:
a: 183.06Å b: 183.06Å c: 198.12Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.21 0.208 0.257
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

4 review citations

Post-transcriptional gene silencing, transcriptional gene silencing and human immunodeficiency virus.
Méndez et al. (2015)
3 more

3 mentions without citation

Structural basis for duplex RNA recognition and cleavage by Archaeoglobus fulgidus C3PO.
Parizotto et al. (2013)
2 more