› 3wtw

X-ray diffraction
2.9Å resolution

Crystal structure of the complex comprised of ETS1(K167A), RUNX1, CBFBETA, and the tcralpha gene enhancer DNA

Released:
DOI: 10.2210/pdb3wtw/pdb
PDB entry 3wtw coloured by chain, front view.
PDB entry 3wtw coloured by chain, side view.
PDB entry 3wtw coloured by chain, top view.
Source organisms:
Primary publication:
A novel allosteric mechanism on protein-DNA interactions underlying the phosphorylation-dependent regulation of Ets1 target gene expressions.
Shiina M, Hamada K, Inoue-Bungo T, Shimamura M, Uchiyama A, Baba S, Sato K, Yamamoto M, Ogata K
J Mol Biol 427 1655-69 (2015)
PMID: 25083921

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-117091 (preferred)
Entry contents:
3 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (5 distinct):
Runt-related transcription factor 1 Chains: A, F
Molecule details ›
Chains: A, F
Length: 204 amino acids
Theoretical weight: 22.86 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03347 (Residues: 60-263; Coverage: 45%)
Gene names: Aml1, Cbfa2, Pebp2ab, Runx1
Sequence domains: Runt domain
Structure domains: Immunoglobulin-like
Core-binding factor subunit beta Chains: B, G
Molecule details ›
Chains: B, G
Length: 142 amino acids
Theoretical weight: 16.68 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q08024 (Residues: 1-142; Coverage: 76%)
Gene names: Cbfb, Pebp2b, Pebpb2
Sequence domains: Core binding factor beta subunit
Structure domains: Core binding factor, beta subunit
Protein C-ets-1 Chains: C, H
Molecule details ›
Chains: C, H
Length: 166 amino acids
Theoretical weight: 19.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P14921 (Residues: 276-441; Coverage: 38%)
Gene names: ETS1, EWSR2
Sequence domains:
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
DNA (5'-D(*GP*AP*AP*GP*CP*CP*AP*CP*AP*TP*CP*CP*TP*CP*T)-3') Chains: D, I
Molecule details ›
Chains: D, I
Length: 15 nucleotides
Theoretical weight: 4.51 KDa
DNA (5'-D(*AP*GP*AP*GP*GP*AP*TP*GP*TP*GP*GP*CP*TP*TP*C)-3') Chains: E, J
Molecule details ›
Chains: E, J
Length: 15 nucleotides
Theoretical weight: 4.67 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P212121
Unit cell:
a: 78.499Å b: 100.613Å c: 194.396Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.219 0.264
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Studies on Molecular Dynamics of Intrinsically Disordered Proteins and Their Fuzzy Complexes: A Mini-Review.
Kasahara et al. (2019)
15 other articles

1 mention without citation

Molecular mechanisms of cooperative binding of transcription factors Runx1-CBFβ-Ets1 on the TCRα gene enhancer.
Kasahara et al. (2017)