› 3wrj

X-ray diffraction
1.85Å resolution

Crystal structure of P450cam

Released:
DOI: 10.2210/pdb3wrj/pdb
PDB entry 3wrj coloured by chain, front view.
PDB entry 3wrj coloured by chain, side view.
PDB entry 3wrj coloured by chain, top view.
Source organism: Pseudomonas putida
Entry authors: Kishimoto A, Takagi K, Amano A, Sakurai K, Mizushima T, Shimada H

Function and Biology Details

Reaction catalysed: 
(+)-camphor + reduced putidaredoxin + O(2) = (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132314 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Camphor 5-monooxygenase Chains: A, E
Molecule details ›
Chains: A, E
Length: 421 amino acids
Theoretical weight: 47.55 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
  • Canonical: P00183 (Residues: 1-415; Coverage: 100%)
Gene names: camC, cyp101
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM
2 bound ligands:
Ligand K 2 x K
Ligand CAM 2 x CAM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P21
Unit cell:
a: 66.885Å b: 62.835Å c: 95.731Å
α: 90° β: 90.83° γ: 90°
R-values:
R R work R free
0.175 0.173 0.221
Expression system: Escherichia coli

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