› 3wou

X-ray diffraction
0.99Å resolution

Crystal Structure of The Recombinant Thaumatin II at 0.99 A

Released:
DOI: 10.2210/pdb3wou/pdb
PDB entry 3wou coloured by chain, front view.
PDB entry 3wou coloured by chain, side view.
PDB entry 3wou coloured by chain, top view.
Source organism: Thaumatococcus daniellii
Primary publication:
Atomic structure of recombinant thaumatin II reveals flexible conformations in two residues critical for sweetness and three consecutive glycine residues.
Masuda T, Mikami B, Tani F
Biochimie 106 33-8 (2014)
PMID: 25066915

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136333 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thaumatin II Chain: A
Molecule details ›
Chain: A
Length: 207 amino acids
Theoretical weight: 22.31 KDa
Source organism: Thaumatococcus daniellii
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P02884 (Residues: 23-229; Coverage: 97%)
Sequence domains: Thaumatin family
Structure domains: Thaumatin

Ligands and Environments

2 bound ligands:
Ligand TLA 2 x TLA
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P41212
Unit cell:
a: 57.685Å b: 57.685Å c: 150.011Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.097 0.097 0.118
Expression system: Komagataella pastoris

Quick links

Citations

1 review citation

Bioproduction of the Recombinant Sweet Protein Thaumatin: Current State of the Art and Perspectives.
Joseph et al. (2019)
2 other articles

1 mention without citation

The oxygen-oxygen distance of water in crystallographic data sets.
Palese LL. (2020)