› 3wh9

X-ray diffraction
1.57Å resolution

The ligand-free structure of ManBK from Aspergillus niger BK01

Released:
DOI: 10.2210/pdb3wh9/pdb
PDB entry 3wh9 coloured by chain, front view.
PDB entry 3wh9 coloured by chain, side view.
PDB entry 3wh9 coloured by chain, top view.
Source organism: Aspergillus niger
Entry authors: Huang JW, Chen CC, Huang CH, Huang TY, Wu TH, Cheng YS, Ko TP, Lin CY, Liu JR, Guo RT

Function and Biology Details

Reaction catalysed: 
Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106928 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Probable mannan endo-1,4-beta-mannosidase A Chains: A, B
Molecule details ›
Chains: A, B
Length: 345 amino acids
Theoretical weight: 37.69 KDa
Source organism: Aspergillus niger
Expression system: Komagataella pastoris
UniProt:
  • Canonical: A2QKT4 (Residues: 39-383; Coverage: 95%)
Gene names: An05g01320, man1, manA
Sequence domains: Cellulase (glycosyl hydrolase family 5)
Structure domains: Glycosidases

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
3 bound ligands:
Ligand NAG 2 x NAG
Ligand TRS 2 x TRS
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13C1
Spacegroup: C2221
Unit cell:
a: 93.584Å b: 97.051Å c: 147.838Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.159 0.19
Expression system: Komagataella pastoris

Quick links