› 3we3

X-ray diffraction
2.9Å resolution

Structure of BLM RQC domain bound to an arsenate ion

Released:
DOI: 10.2210/pdb3we3/pdb
PDB entry 3we3 coloured by chain, front view.
PDB entry 3we3 coloured by chain, side view.
PDB entry 3we3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the RecQ C-terminal domain of human Bloom syndrome protein.
Kim SY, Hakoshima T, Kitano K
Sci Rep 3 3294 (2013)
PMID: 24257077

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156943 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RecQ-like DNA helicase BLM Chains: A, B
Molecule details ›
Chains: A, B
Length: 147 amino acids
Theoretical weight: 16.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P54132 (Residues: 1068-1209; Coverage: 10%)
Gene names: BLM, RECQ2, RECQL3
Sequence domains: RQC domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

2 bound ligands:
Ligand ART 1 x ART
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P43212
Unit cell:
a: 59.717Å b: 59.717Å c: 209.678Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.269 0.269 0.295
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Bloom's Syndrome: Clinical Spectrum, Molecular Pathogenesis, and Cancer Predisposition.
Cunniff et al. (2017)
2 more

1 mention without citation

Structure of the RecQ C-terminal domain of human Bloom syndrome protein.
Kim et al. (2013)