› 3w3k

X-ray diffraction
2.3Å resolution

Crystal structure of human TLR8 in complex with CL075

Released:
DOI: 10.2210/pdb3w3k/pdb
PDB entry 3w3k coloured by chain, front view.
PDB entry 3w3k coloured by chain, side view.
PDB entry 3w3k coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural reorganization of the Toll-like receptor 8 dimer induced by agonistic ligands.
Tanji H, Ohto U, Shibata T, Miyake K, Shimizu T
Science 339 1426-9 (2013)
PMID: 23520111

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192550 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Toll-like receptor 8 Chains: A, B
Molecule details ›
Chains: A, B
Length: 811 amino acids
Theoretical weight: 92.84 KDa
Source organism: Homo sapiens
Expression system: Drosophila
UniProt:
  • Canonical: Q9NR97 (Residues: 27-827; Coverage: 79%)
Gene names: TLR8, UNQ249/PRO286
Sequence domains: Leucine rich repeat
Structure domains: Ribonuclease Inhibitor

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand L07 2 x L07
Ligand NAG 6 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21
Unit cell:
a: 72.41Å b: 154.02Å c: 86.86Å
α: 90° β: 103.03° γ: 90°
R-values:
R R work R free
0.207 0.205 0.244
Expression system: Drosophila

Quick links

Citations

65 review citations

Toll-like receptor signaling pathways.
Kawasaki et al. (2014)
64 more

11 mentions without citation

Structural and functional understanding of the toll-like receptors.
Asami et al. (2021)
10 more