› 3vu8

X-ray diffraction
2.2Å resolution

Metionyl-tRNA synthetase from Thermus thermophilus complexed with methionyl-adenylate analogue

Released:
DOI: 10.2210/pdb3vu8/pdb
PDB entry 3vu8 coloured by chain, front view.
PDB entry 3vu8 coloured by chain, side view.
PDB entry 3vu8 coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry authors: Konno M, Kato-Murayama M, Toma-Fukai S, Uchikawa E, Nureki O, Yokoyama S

Function and Biology Details

Reaction catalysed: 
ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149951 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methionine--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 502 amino acids
Theoretical weight: 58.22 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: P23395 (Residues: 1-502; Coverage: 81%)
Gene names: TTHA1298, metG, metS
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand MDS 1 x MDS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 50.823Å b: 82.682Å c: 120.165Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.2 0.3
Expression system: Escherichia coli

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