› 3vgw

X-ray diffraction
1.6Å resolution

Crystal structure of monoAc-biotin-avidin complex

Released:
DOI: 10.2210/pdb3vgw/pdb
PDB entry 3vgw coloured by chain, front view.
PDB entry 3vgw coloured by chain, side view.
PDB entry 3vgw coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Rational development of caged-biotin protein-labeling agents and some applications in live cells.
Terai T, Maki E, Sugiyama S, Takahashi Y, Matsumura H, Mori Y, Nagano T
Chem Biol 18 1261-72 (2011)
PMID: 22035795

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136083 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Avidin Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 123 amino acids
Theoretical weight: 13.72 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P02701 (Residues: 25-147; Coverage: 96%)
Gene name: AVD
Sequence domains: Avidin family
Structure domains: Avidin-like

Ligands and Environments

3 bound ligands:
Ligand NVZ 8 x NVZ
Ligand NAG 8 x NAG
Ligand SO4 12 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P21
Unit cell:
a: 116.01Å b: 81.224Å c: 74.41Å
α: 90° β: 92.47° γ: 90°
R-values:
R R work R free
0.191 0.19 0.215

Quick links

Citations

1 review citation

Engineering Proteins at Interfaces: From Complementary Characterization to Material Surfaces with Designed Functions.
Morsbach et al. (2018)
7 other articles

1 mention without citation

Structural Characterization of the Avidin Interactions with Fluorescent Pyrene-Conjugates: 1-Biotinylpyrene and 1-Desthiobiotinylpyrene.
Strzelczyk et al. (2016)