› 3ve7

X-ray diffraction
1.54Å resolution

Crystal structure of orotidine 5'-monophosphate decarboxylase from Metallosphaera sedula complexed with inhibitor BMP

Released:
DOI: 10.2210/pdb3ve7/pdb
PDB entry 3ve7 coloured by chain, front view.
PDB entry 3ve7 coloured by chain, side view.
PDB entry 3ve7 coloured by chain, top view.
Source organism: Metallosphaera sedula DSM 5348
Entry authors: Fedorov AA, Fedorov EV, Desai B, Gerlt JA, Almo SC

Function and Biology Details

Reaction catalysed: 
Orotidine 5'-phosphate = UMP + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107506 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
orotidine-5'-phosphate decarboxylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 215 amino acids
Theoretical weight: 23.43 KDa
Source organism: Metallosphaera sedula DSM 5348
Expression system: Escherichia coli
UniProt:
  • Canonical: A4YI54 (Residues: 1-215; Coverage: 100%)
Gene name: Msed_1966
Sequence domains: Orotidine 5'-phosphate decarboxylase / HUMPS family
Structure domains: Aldolase class I

Ligands and Environments

3 bound ligands:
Ligand BMP 2 x BMP
Ligand SO4 3 x SO4
Ligand ACY 3 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 41.396Å b: 91.57Å c: 50.954Å
α: 90° β: 98.79° γ: 90°
R-values:
R R work R free
0.17 0.169 0.201
Expression system: Escherichia coli

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