› 3v46

X-ray diffraction
1.55Å resolution

Crystal Structure of Yeast Cdc73 C-Terminal Domain

Released:
DOI: 10.2210/pdb3v46/pdb
PDB entry 3v46 coloured by chain, front view.
PDB entry 3v46 coloured by chain, side view.
PDB entry 3v46 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Cdc73 subunit of Paf1 complex contains C-terminal Ras-like domain that promotes association of Paf1 complex with chromatin.
Amrich CG, Davis CP, Rogal WP, Shirra MK, Heroux A, Gardner RG, Arndt KM, VanDemark AP
J Biol Chem 287 10863-75 (2012)
PMID: 22318720

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170378 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cell division control protein 73 Chain: A
Molecule details ›
Chain: A
Length: 170 amino acids
Theoretical weight: 20.07 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q06697 (Residues: 230-393; Coverage: 42%)
Gene names: CDC73, YLR418C
Sequence domains: RNA pol II accessory factor, Cdc73 family, C-terminal
Structure domains: RNA polymerase II accessory factor, Cdc73 C-terminal domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P41212
Unit cell:
a: 53.324Å b: 53.324Å c: 130.223Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.172 0.21
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Emerging Insights into the Roles of the Paf1 Complex in Gene Regulation.
Van Oss et al. (2017)
2 more

6 mentions without citation

The Paf1 Complex: A Keystone of Nuclear Regulation Operating at the Interface of Transcription and Chromatin.
Francette et al. (2021)
5 more