› 3uwb

X-ray diffraction
1.7Å resolution

Crystal structure of a probable peptide deformylase from strucynechococcus phage S-SSM7 in complex with actinonin

Released:
DOI: 10.2210/pdb3uwb/pdb
PDB entry 3uwb coloured by chain, front view.
PDB entry 3uwb coloured by chain, side view.
PDB entry 3uwb coloured by chain, top view.
Source organism: Synechococcus phage S-SSM7
Primary publication:
Structure and function of a cyanophage-encoded peptide deformylase.
Frank JA, Lorimer D, Youle M, Witte P, Craig T, Abendroth J, Rohwer F, Edwards RA, Segall AM, Burgin AB
ISME J 7 1150-60 (2013)
PMID: 23407310

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122906 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RIIA-RIIB membrane-associated protein Chain: A
Molecule details ›
Chain: A
Length: 154 amino acids
Theoretical weight: 17.84 KDa
Source organism: Synechococcus phage S-SSM7
Expression system: Escherichia coli
UniProt:
  • Canonical: E3SLL2 (Residues: 1-128; Coverage: 100%)
Gene name: SSSM7_299
Sequence domains: Polypeptide deformylase
Structure domains: Peptide deformylase

Ligands and Environments

4 bound ligands:
Ligand ZN 1 x ZN
Ligand CL 1 x CL
Ligand EDO 1 x EDO
Ligand BB2 1 x BB2
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 47.81Å b: 59.15Å c: 61.46Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.155 0.171
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Phage puppet masters of the marine microbial realm.
Breitbart et al. (2018)
4 more

1 mention without citation

Structure and function of a cyanophage-encoded peptide deformylase.
Frank et al. (2013)