› 3uoi

X-ray diffraction
1.9Å resolution

Mycobacterium tuberculosis bacterioferritin, BfrA

Released:
DOI: 10.2210/pdb3uoi/pdb
PDB entry 3uoi coloured by chain, front view.
PDB entry 3uoi coloured by chain, side view.
PDB entry 3uoi coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Entry authors: McMath LM, Goulding CW, TB Structural Genomics Consortium (TBSGC)

Function and Biology Details

Reaction catalysed: 
4 Fe(2+) + 4 H(+) + O(2) = 4 Fe(3+) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo 24-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162008 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacterioferritin BfrA Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X, a, b, c, d, e, f, g, h, i, j, k, l, m, n, o, p, q, r, s, t, u, v, w, x
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X, a, b, c, d, e, f, g, h, i, j, k, l, m, n, o, p, q, r, s, t, u, v, w, x
Length: 161 amino acids
Theoretical weight: 18.55 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WPQ9 (Residues: 1-159; Coverage: 100%)
Gene names: MTCY180.42c, Rv1876, bfr, bfrA
Sequence domains: Ferritin-like domain
Structure domains: Ferritin

Ligands and Environments

1 bound ligand:
Ligand HEM 24 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P1
Unit cell:
a: 122.962Å b: 123.225Å c: 175.452Å
α: 89.95° β: 89.95° γ: 90°
R-values:
R R work R free
0.151 0.148 0.194
Expression system: Escherichia coli BL21(DE3)

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