› 3ulu

X-ray diffraction
3.52Å resolution

Structure of quaternary complex of human TLR3ecd with three Fabs (Form1)

Released:
DOI: 10.2210/pdb3ulu/pdb
PDB entry 3ulu coloured by chain, front view.
PDB entry 3ulu coloured by chain, side view.
PDB entry 3ulu coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Lateral clustering of TLR3:dsRNA signaling units revealed by TLR3ecd:3Fabs quaternary structure.
Luo J, Obmolova G, Malia TJ, Wu SJ, Duffy KE, Marion JD, Bell JK, Ge P, Zhou ZH, Teplyakov A, Zhao Y, Lamb RJ, Jordan JL, San Mateo LR, Sweet RW, Gilliland GL
J Mol Biol 421 112-24 (2012)
PMID: 22579623

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208051 (preferred)
Entry contents:
7 distinct polypeptide molecules
Macromolecules (10 distinct):
Toll-like receptor 3 Chain: A
Molecule details ›
Chain: A
Length: 694 amino acids
Theoretical weight: 78.79 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O15455 (Residues: 22-702; Coverage: 77%)
Gene name: TLR3
Sequence domains:
Structure domains: Ribonuclease Inhibitor
Fab15 light chain Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.24 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Fab15 heavy chain Chain: H
Molecule details ›
Chain: H
Length: 225 amino acids
Theoretical weight: 24.46 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Fab12 light chain Chain: C
Molecule details ›
Chain: C
Length: 213 amino acids
Theoretical weight: 22.75 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Fab12 heavy chain Chain: D
Molecule details ›
Chain: D
Length: 226 amino acids
Theoretical weight: 24.58 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Fab1068 light chain Chain: E
Molecule details ›
Chain: E
Length: 215 amino acids
Theoretical weight: 23.61 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Fab1068 heavy chain Chain: F
Molecule details ›
Chain: F
Length: 223 amino acids
Theoretical weight: 24.3 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
2 bound ligands:
Ligand NAG 5 x NAG
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2
Unit cell:
a: 214.9Å b: 142.08Å c: 125.04Å
α: 90° β: 103.17° γ: 90°
R-values:
R R work R free
0.27 0.268 0.309
Expression systems:
  • Spodoptera frugiperda
  • Homo sapiens

Quick links

Citations

10 review citations

Assembly and localization of Toll-like receptor signalling complexes.
Gay et al. (2014)
9 more

7 mentions without citation

Toll-Like Receptors: General Molecular and Structural Biology.
Behzadi et al. (2021)
6 more