› 3ul0

X-ray diffraction
2Å resolution

Mouse importin alpha: mouse CBP80Y8D cNLS complex

Released:
DOI: 10.2210/pdb3ul0/pdb
PDB entry 3ul0 coloured by chain, front view.
PDB entry 3ul0 coloured by chain, side view.
PDB entry 3ul0 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis of high-affinity nuclear localization signal interactions with importin-α.
Marfori M, Lonhienne TG, Forwood JK, Kobe B
Traffic 13 532-48 (2012)
PMID: 22248489

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156525 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Importin subunit alpha-1 Chain: B
Molecule details ›
Chain: B
Length: 510 amino acids
Theoretical weight: 55.27 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P52293 (Residues: 70-529; Coverage: 87%)
Gene names: Kpna2, Rch1
Sequence domains:
Structure domains: Leucine-rich Repeat Variant
Nuclear cap-binding protein subunit 1 Chain: C
Molecule details ›
Chain: C
Length: 25 amino acids
Theoretical weight: 2.9 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q3UYV9 (Residues: 1-23; Coverage: 3%)
Gene names: Cbp80, Ncbp1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P212121
Unit cell:
a: 78.691Å b: 89.898Å c: 99.792Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.188 0.208
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Broad targeting of resistance to apoptosis in cancer.
Mohammad et al. (2015)
8 more

1 mention without citation

Design rules for selective binding of nuclear localization signals to minor site of importin α.
Pang et al. (2014)