› 3ugl

X-ray diffraction
1.36Å resolution

Structural and functional characterization of an anesthetic binding site in the second cysteine-rich domain of protein kinase C delta

Released:
DOI: 10.2210/pdb3ugl/pdb
PDB entry 3ugl coloured by chain, front view.
PDB entry 3ugl coloured by chain, side view.
PDB entry 3ugl coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural and functional characterization of an anesthetic binding site in the second cysteine-rich domain of protein kinase Cδ*.
Shanmugasundararaj S, Das J, Sandberg WS, Zhou X, Wang D, Messing RO, Bruzik KS, Stehle T, Miller KW
Biophys J 103 2331-40 (2012)
PMID: 23283232

Function and Biology Details

Reactions catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151301 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein kinase C delta type regulatory subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 65 amino acids
Theoretical weight: 7.38 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P28867 (Residues: 231-280; Coverage: 7%)
Gene names: Pkcd, Prkcd
Sequence domains: Phorbol esters/diacylglycerol binding domain (C1 domain)
Structure domains: Wheat Germ Agglutinin (Isolectin 2); domain 1

Ligands and Environments

3 bound ligands:
Ligand ZN 4 x ZN
Ligand 09V 2 x 09V
Ligand PO4 3 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P21
Unit cell:
a: 43.876Å b: 32.522Å c: 49.816Å
α: 90° β: 94.02° γ: 90°
R-values:
R R work R free
0.148 0.147 0.17
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Dynamics and Membrane Interactions of Protein Kinase C.
Igumenova TI. (2015)
3 more

1 mention without citation

Structural and functional characterization of an anesthetic binding site in the second cysteine-rich domain of protein kinase Cδ*.
Shanmugasundararaj et al. (2012)