› 3uce

X-ray diffraction
1.8Å resolution

Crystal structure of a small-chain dehydrogenase in complex with NADPH

Released:
DOI: 10.2210/pdb3uce/pdb
PDB entry 3uce coloured by chain, front view.
PDB entry 3uce coloured by chain, side view.
PDB entry 3uce coloured by chain, top view.
Source organism: Vibrio vulnificus YJ016
Primary publication:
Structural and biochemical characterization of an atypical short-chain dehydrogenase/reductase reveals an unusual cofactor preference.
Buysschaert G, Verstraete K, Savvides SN, Vergauwen B
FEBS J 280 1358-70 (2013)
PMID: 23311896

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181717 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 223 amino acids
Theoretical weight: 23.68 KDa
Source organism: Vibrio vulnificus YJ016
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7MBY8 (Residues: 1-223; Coverage: 100%)
Gene name: VVA1599
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

1 bound ligand:
Ligand NDP 4 x NDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 69.63Å b: 96.26Å c: 72.11Å
α: 90° β: 103.99° γ: 90°
R-values:
R R work R free
0.177 0.175 0.219
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Recent developments in the use of differential scanning fluorometry in protein and small molecule discovery and characterization.
Simeonov A. (2013)
7 other articles