› 3uc1

X-ray diffraction
1.65Å resolution

Mycobacterium tuberculosis gyrase type IIA topoisomerase C-terminal domain

Released:
DOI: 10.2210/pdb3uc1/pdb
PDB entry 3uc1 coloured by chain, front view.
PDB entry 3uc1 coloured by chain, side view.
PDB entry 3uc1 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Mechanisms for defining supercoiling set point of DNA gyrase orthologs: II. The shape of the GyrA subunit C-terminal domain (CTD) is not a sole determinant for controlling supercoiling efficiency.
Tretter EM, Berger JM
J Biol Chem 287 18645-54 (2012)
PMID: 22457352

Function and Biology Details

Reaction catalysed: 
ATP-dependent breakage, passage and rejoining of double-stranded DNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161456 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA gyrase subunit A Chain: A
Molecule details ›
Chain: A
Length: 327 amino acids
Theoretical weight: 35.29 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WG47 (Residues: 514-838; Coverage: 39%)
Gene names: MTCY10H4.04, Rv0006, gyrA
Sequence domains: DNA gyrase C-terminal domain, beta-propeller
Structure domains: DNA gyrase/topoisomerase IV, subunit A, C-terminal

Ligands and Environments

3 bound ligands:
Ligand CA 1 x CA
Ligand ACT 2 x ACT
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 38.871Å b: 82.842Å c: 83.433Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.188 0.222
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Mechanism of quinolone action and resistance.
Aldred et al. (2014)
9 more

3 mentions without citation

β-Propeller blades as ancestral peptides in protein evolution.
Kopec et al. (2013)
2 more