› 3uag

X-ray diffraction
1.77Å resolution

UDP-N-ACETYLMURAMOYL-L-ALANINE:D-GLUTAMATE LIGASE

Released:
DOI: 10.2210/pdb3uag/pdb
PDB entry 3uag coloured by chain, front view.
PDB entry 3uag coloured by chain, side view.
PDB entry 3uag coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Determination of the MurD mechanism through crystallographic analysis of enzyme complexes.
Bertrand JA, Auger G, Martin L, Fanchon E, Blanot D, Le Beller D, van Heijenoort J, Dideberg O
J Mol Biol 289 579-90 (1999)
PMID: 10356330

Function and Biology Details

Reaction catalysed: 
ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanine + D-glutamate = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147132 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetylmuramoylalanine--D-glutamate ligase Chain: A
Molecule details ›
Chain: A
Length: 437 amino acids
Theoretical weight: 46.93 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P14900 (Residues: 2-438; Coverage: 100%)
Gene names: JW0086, b0088, murD
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand MN 1 x MN
Ligand UMA 1 x UMA
Ligand ADP 1 x ADP
Ligand EPE 1 x EPE
1 modified residue:
Ligand KCX 1 x KCX

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P41
Unit cell:
a: 65.24Å b: 65.24Å c: 134.41Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.178 0.222
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Cytoplasmic steps of peptidoglycan biosynthesis.
Barreteau et al. (2008)
12 more

8 mentions without citation

From the similarity analysis of protein cavities to the functional classification of protein families using cavbase.
Kuhn et al. (2006)
7 more