3u61

X-ray diffraction
3.2Å resolution

Structure of T4 Bacteriophage Clamp Loader Bound To Closed Clamp, DNA and ATP Analog and ADP

Released:
Source organism: Tequatrovirus T4
Primary publication:
How a DNA polymerase clamp loader opens a sliding clamp.
Science 334 1675-80 (2011)
PMID: 22194570

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116961 (preferred)
Entry contents:
3 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (5 distinct):
Sliding-clamp-loader large subunit Chains: B, C, D, E
Molecule details ›
Chains: B, C, D, E
Length: 324 amino acids
Theoretical weight: 36.19 KDa
Source organism: Tequatrovirus T4
Expression system: Escherichia coli
UniProt:
  • Canonical: P04526 (Residues: 1-319; Coverage: 100%)
Gene name: 44
Sequence domains:
Structure domains:
Sliding-clamp-loader small subunit Chain: A
Molecule details ›
Chain: A
Length: 199 amino acids
Theoretical weight: 22.94 KDa
Source organism: Tequatrovirus T4
Expression system: Escherichia coli
UniProt:
  • Canonical: P04527 (Residues: 2-187; Coverage: 100%)
Gene name: 62
Sequence domains: Bacteriophage clamp loader A subunit
Structure domains:
Sliding clamp Chains: F, G, H
Molecule details ›
Chains: F, G, H
Length: 228 amino acids
Theoretical weight: 25.16 KDa
Source organism: Tequatrovirus T4
Expression system: Escherichia coli
UniProt:
  • Canonical: P04525 (Residues: 1-228; Coverage: 100%)
Gene name: 45
Sequence domains:
Structure domains: Proliferating Cell Nuclear Antigen
Template DNA strand Chain: I
Molecule details ›
Chain: I
Length: 20 nucleotides
Theoretical weight: 6.12 KDa
Primer DNA strand Chain: J
Molecule details ›
Chain: J
Length: 10 nucleotides
Theoretical weight: 3.01 KDa

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P212121
Unit cell:
a: 82.303Å b: 137.272Å c: 222.779Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.244 0.239 0.305
Expression system: Escherichia coli