› 3u3g

X-ray diffraction
1.4Å resolution

Structure of LC11-RNase H1 Isolated from Compost by Metagenomic Approach: Insight into the Structural Bases for Unusual Enzymatic Properties of Sto-RNase H1

Released:
DOI: 10.2210/pdb3u3g/pdb
PDB entry 3u3g coloured by chain, front view.
PDB entry 3u3g coloured by chain, side view.
PDB entry 3u3g coloured by chain, top view.
Source organism: uncultured organism
Primary publication:
Activity, stability, and structure of metagenome-derived LC11-RNase H1, a homolog of Sulfolobus tokodaii RNase H1.
Nguyen TN, Angkawidjaja C, Kanaya E, Koga Y, Takano K, Kanaya S
Protein Sci 21 553-61 (2012)
PMID: 22389131

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122727 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNase H type-1 domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 140 amino acids
Theoretical weight: 15.63 KDa
Source organism: uncultured organism
Expression system: Escherichia coli
UniProt:
  • Canonical: E0X767 (Residues: 1-140; Coverage: 100%)
Sequence domains: Reverse transcriptase-like
Structure domains: Ribonuclease H-like superfamily/Ribonuclease H

Ligands and Environments

2 bound ligands:
Ligand CL 2 x CL
Ligand UNL 1 x UNL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P21
Unit cell:
a: 54.315Å b: 104.891Å c: 57.212Å
α: 90° β: 110.34° γ: 90°
R-values:
R R work R free
0.173 0.172 0.192
Expression system: Escherichia coli

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Citations

7 citation in other articles

Role of RNase H1 in DNA repair: removal of single ribonucleotide misincorporated into DNA in collaboration with RNase H2.
Tannous et al. (2015)
6 more

1 mention without citation

Activity, stability, and structure of metagenome-derived LC11-RNase H1, a homolog of Sulfolobus tokodaii RNase H1.
Nguyen et al. (2012)